Journal: International journal of molecular sciences

Article Title: Cellular and Molecular Variations in Male and Female Murine Skeletal Muscle after Long-Term Feeding with a High-Fat Diet.

doi: 10.3390/ijms23179547

Figure Lengend Snippet: Figure 4. Immunocytochemical labeling for different fiber types within the tibialis anterior muscle of control (A–D) and high-fat diet-fed (E–H) male mice. The figure shows immunolabeling for type I fibers (A,E), immunolabeling for type IIA fibers (B,F), immunolabeling for type IIX fibers (C,G), and immunolabeling for type IIB fibers (D,H). There were no type I slow fibers, whereas the most frequent fibers were type IIB. Scale bar = 30 µm.

Article Snippet: Primary Antibodies The following mouse monoclonal primary antibodies were used to determine the fiber type and size: (1) BF-F3 from the Developmental Studies Hybridoma Bank (DSHB; 1:50 dilution) to detect myosin heavy chain type IIb (fast-twitch glycolytic); (2) SC-71 (DSHB; 1:50 dilution) to detect myosin heavy chain type IIA (fast-twitch oxidative glycolytic); (3) 6H1 (DSHB; 1:50 dilution) to detect myosin heavy chain type IIX (fast-twitch glycolytic); (4) BAF8 (DSHB; 1:50 dilution) to detect myosin heavy chain type I (slow-twitch oxidative) [37]. (5) We also used a rabbit polyclonal anti-laminin primary antibody (L9393; Sigma-Aldrich, St. Louis, MO, USA) at a 1:400 dilution to detect the basal laminae of skeletal muscle fibers [38].

Techniques: Labeling, Control, Immunolabeling

Journal: International journal of molecular sciences

Article Title: Cellular and Molecular Variations in Male and Female Murine Skeletal Muscle after Long-Term Feeding with a High-Fat Diet.

doi: 10.3390/ijms23179547

Figure Lengend Snippet: Figure 8. RT-qPCR of (A) male and (B) female tibialis anterior muscle revealed a reduction in the expression of muscle fiber-specific genes Myh1 (IIX) and Myh2 (IIA) in both sexes. An increase in Myh4 (IIB) was seen in males, while there was no significant increase in females. Pax7 expression was reduced in both males and females, while Mstn expression was increased in both sexes. * p < 0.05, ** p < 0.01, *** p < 0.001 significantly different from its corresponding control group (t-test).

Article Snippet: Primary Antibodies The following mouse monoclonal primary antibodies were used to determine the fiber type and size: (1) BF-F3 from the Developmental Studies Hybridoma Bank (DSHB; 1:50 dilution) to detect myosin heavy chain type IIb (fast-twitch glycolytic); (2) SC-71 (DSHB; 1:50 dilution) to detect myosin heavy chain type IIA (fast-twitch oxidative glycolytic); (3) 6H1 (DSHB; 1:50 dilution) to detect myosin heavy chain type IIX (fast-twitch glycolytic); (4) BAF8 (DSHB; 1:50 dilution) to detect myosin heavy chain type I (slow-twitch oxidative) [37]. (5) We also used a rabbit polyclonal anti-laminin primary antibody (L9393; Sigma-Aldrich, St. Louis, MO, USA) at a 1:400 dilution to detect the basal laminae of skeletal muscle fibers [38].

Techniques: Quantitative RT-PCR, Expressing, Control

Journal: Pathogens

Article Title: High-Throughput Sequencing, a Versatile Weapon to Support Genome-Based Diagnosis in Infectious Diseases: Applications to Clinical Bacteriology

doi: 10.3390/pathogens3020258

Figure Lengend Snippet: Innovative clinical investigations providing findings for a future implementation of high-throughput sequencing (HTS) in genome-based diagnosis. WGS, whole genome sequencing; MLST, multi locus sequence typing.

Article Snippet: Immunomagnetic separation for targeted bacterial enrichment with multiple displacement amplification , C. trachomatis /cervical or vaginal swab , retrospective , Illumina GA IIx and HiSeq , [ ] .

Techniques: Next-Generation Sequencing, Sequencing, Isolation, Immunomagnetic Separation, Multiple Displacement Amplification